Regulatory

Part:BBa_K1634002:Design

Designed by: Xinhong Chen   Group: iGEM15_China_Tongji   (2015-09-13)


pmyo2 ( a promoter in C.elegans )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 288
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 198


Design Notes

Since we use the restriction enzyme site to do the ligation, we should pay attention to the sequence of Pmyo2, avoiding using the restriction site exits in it.


Source

We get Pmyo2 from the C.elegans' genomic DNA by PCR.

References